Advanced Water & Waste Water Quality Control Lab
The Advanced Water and Wastewater Quality Laboratory Lab (ADWWQL) at USPCASW is Sind EPA accredited lab and provides commercialized sample testing services on various drinking and waste water samples by following guidelines of WHO, EPA and NEQS recommended standard methods. The ADWWQL is providing services for analyses of physical, chemical, and biological parameters (PCB) of surface, river, ground, water treatment plants, industrial effluents, sewerage, sources. Our labs also provide services of sample collector to visitor /outsider if he or she wants to collect samples from everywhere in Sindh or other nearby areas of Sindh by following proper safety protocols.
Most of our Graduates scholars (MS and Ph.D.) working on developing new innovative methods of treating contaminated water, and wastewater reuse on a lab/pilot scale such as advanced nanotechnology, molecular biology (membrane filters, nanocatalyst, absorbent (biochar), sensors, and membrane bioreactor, disinfectant and drug-resistant bacteria and antibiotic-resistant genes. The laboratory also provides training in environment-related analytical Instrumental laboratory techniques, Health and Safety Laboratory Protocols, Design and Development of Water and Wastewater Treatment, Advanced Water and Wastewater Quality Parameters including Physical, Chemical, and Biological.
The Environmental Engineering Department comprises two research laboratories: the Advanced Water and Wastewater Quality Control Laboratory (Lab No. 04) and the Pilot-Scale Water and Wastewater Treatment Laboratory (Lab No. 02). Both laboratories are adequately equipped to conduct laboratory-based as well as on-site/field research activities required by students and faculty members.
All laboratories maintain complete documentation, including equipment manuals, work instructions, laboratory safety protocols, maintenance log sheets, student attendance records, and commercialized water testing Standard Operating Procedures (SOPs) covering all SEPA drinking water and wastewater quality parameters. In addition, quotations, invoices, testing report records, and an updated rate list for all water, wastewater, air, and noise testing parameters are available on the department’s website.
Objectives
The primary objective of the Advanced Water and Wastewater Quality Control Laboratories is to provide hands-on practical training and applied research opportunities in support of prescribed academic courses and national water sector needs.
- The laboratories focus on applied research addressing critical challenges in Pakistan’s water sector, including industrial wastewater management, water reuse, and sustainable treatment technologies. Emphasis is placed on human resource development, technology innovation, academia–industry collaboration, and policy formulation. The Center works closely with industries in Karachi and surrounding regions, particularly textile and sugar industries, to enhance water-use efficiency and wastewater treatment practices.
- A major goal of USPCAS-W is alignment with the United Nations Sustainable Development Goal (SDG) 6, targeting indicators 6.1, 6.2, 6.3, 6.4, 6.5, 6.6, 6.a, and 6.b by 2030. The laboratories conduct comprehensive water and wastewater quality analyses of surface water, rivers, groundwater, water treatment plants, industrial effluents, and sewerage systems, covering physical, chemical, and biological parameters.
- The laboratories emphasize the development of innovative techniques and technologies for the detection, treatment, removal, and mitigation of environmental pollutants. Research activities utilize advanced analytical instrumentation available at USPCAS-W, MUET, Jamshoro, to improve overall water quality and environmental sustainability.
- USPCAS-W laboratories provide commercialized water and wastewater testing services in accordance with WHO, EPA, and NEQS standard methods. An updated rate list for all water quality testing parameters is available on the official website. Sample collection services are also offered across Sindh and nearby regions, following approved safety and sampling protocols. All analyses culminate in authenticated test reports issued against formal requisitions.
- Graduate scholars (MS and PhD) are actively engaged in research on advanced water and wastewater treatment and reuse technologies at laboratory and pilot scale, including nanotechnology, membrane filtration, nanocatalysts, biochar adsorption, sensors, membrane bioreactors, molecular microbiology, disinfectants, drug-resistant bacteria, and antibiotic-resistant genes.
- The laboratories also provide specialized training in:
- Environmental analytical and instrumental techniques
- Laboratory health and safety protocols
- Design and development of water and wastewater treatment systems
- Advanced water and wastewater quality parameters, including physical, chemical, and biological indicators such as pH, temperature, color, odor, hardness, alkalinity, chloride, sulfate, nitrate, fluoride, TDS, EC, salinity, BOD, COD, TSS, total coliforms, and E. coli
- Field water sampling protocols (collection, preservation, and transportation)
- Antibiotic-resistant bacteria and basic molecular microbiology
- Experimental design and research assessment
- Intership facility to department level and also for other organizations
| Major Equipment present in the Labs | ||
| S.No | Name of Equipment | |
| 1 | DOUBLE BEAM UV/VISIBLE SPECTROPHOTOMETER Lambda 365 | |
| 2 | Water Distillation Apparatus Model: BMS-4CE Biotechnology Medical Services K. Canada In | |
| 3 | Total Organic Carbon Analyzer High Sensitive Stand Alone Model TOC-L CSH | |
| 4 | Prominence Ion Chromatograph HIC-NS | |
| 5 | Water Logger 55.496.001.G.A Water Logger/ Multiparameter probe HL4 | |
| 6 | EPI Fluorescent Microscope ZEISS Axio Scope. A1 Fluorescence Microscope | |
| 7 | High Speed Refrigerated Centrifuge Machine, Model Z36HK, Hermle Made in Germany | |
| 8 | Dissolved Oxygen Meter Model Multi 9430 Set KWTW Made in Germany | |
| 9 | Model Multi 3430, Cat No. 2FD47F WTW, Made in Germany | |
| 10 | Ultra Low Temperature Freezer Model MDF-U33V, Panasonic (New Name of Sanyo) Made in Japan | |
| 11 | Orbital Shaker Digital Model Unimax 2010, Heidolph Made in Germany | |
| 12 | Universal Oven Model UN-30, Memmert, Made in Germany | |
| 13 | PH Meter Digital Bench Model | |
| 14 | pH meter Portable | |
| 15 | Conductivity Meter Digital Bench Top Microprocessor Model Inolab Cond 7110 WTW Made in Germany | |
| 16 | Conductivity Meter Portable Model Cond 3110, WTW Made in Germany | |
| 17 | Laminar Flow Cabinet Model SCV-4AX, Streamline, Singapore | |
| 18 | BOD Incubator Model FOC 120E , Velp Made in Italy | |
| 19 | Muffle Furnace Model JFF 2000 DENTSPLY CERAMCO Made in USA | |
| 20 | ULTRA PURELAB Ultra Analytic | |
| 21 | Hight Temperature Chamber Furnace Model # HTF 18/4 | |
| 22 | Turbidity Meters Turbicheck- Tintometer, GmbH Benchtop | |
| 23 | HACH 2100Q Portable Turbiditimeter (WR795-20) | |
| 24 | Ambient SO2 Monitor/ Gas Detector- IX 176-SO2, IMR Environmental Equipment, USA. | |
| 25 | Digital Sound Level Meter, Testo 815, Testo AG of Germany | |
| 26 | Particulate PM2.5 & PM10 Monitor, EPAM-5000, Environmental Devices Corporation, USA | |
| 27 | Electronic Balances | |
| 28 | Water Baths, OPTIMA SERIES BJ186-36 THERMOSTATIC BATH GRANT | |
| 29 | CO and CO2 Analyzer AQ COMFORT-4 | |
| 30 | Ambient NO2 Monitor Z-1400 XP | |
| 31 | Inductively Coupled Plasma- Mass Spectrometer (Multitype ICP Emission Spectrometer) Make: Perkin Elmer Model: NexION 350Q ICPMS System | |
| 32 | Microwave Digester Make: CEM, International Model: Mars 6iWave | |
| 33 | Real-Time PCR Machine Make: Analytikjena Model: qTower Real Time PCR System | |
| 34 | Micro-pipetts | |
| 35 | Micro centrifuge Labnet Spectrafuge 24D | |
| 36 | Vortex Mixer, IKA-Werke GMBH, MS3 Digital Vortex Mixer | |
| 37 | ProODO BOD Advanced Lab Kit Tintometer Lovibond BD 600- BOD Measurement System | |
| 38 | COD Test apparatus Make: Lovibond/ Tintometer GmbH Model: MD 200 COD VARIO | |
| 39 | GCMS- Shimadzu | |
| 40 | LCMS-Waters | |
| 41 | Electrospinning with multi injection ports | |
| 42 | Liquid Chromatography /Mass Spectrometry (LC-MS/MS) system equipped Water ACQUITY UPL with triple quadrupole MS/MS detector Xevo TQ-S (MASS HUNTER) | |
| 43 | Real Time qPCR | |
| 44 | Pilot Scale Membrane Biological reactors | |
Safety Regulations
The laboratories are fully equipped with comprehensive health, safety, and security systems to ensure a safe working environment for students, faculty, and technical staff. Fire safety measures include firefighting cylinders, CO and CO₂ gas sensors, and sand buckets strategically placed throughout the laboratories. Personal Protective Equipment (PPE) such as lab coats, gloves, safety goggles, face shields, and masks are readily available and mandatory during experimental work.
Access control and security are maintained through RFID card–based entry systems for authorized students, researchers, and staff, along with CCTV surveillance cameras installed to monitor laboratory activities and ensure compliance with safety protocols. Each laboratory is equipped with a fully stocked First Aid box, emergency internal exit doors, and clearly marked emergency evacuation routes.
To mitigate chemical exposure risks, Material Safety Data Sheets (MSDS) for all chemicals are maintained and easily accessible. Dedicated safety showers (eye wash and body wash units) are installed for emergency decontamination. Proper ventilation systems, including exhaust fans and fume extraction units, are provided to control fumes, vapors, and odors during experimental procedures.
All analytical instruments are supported by uninterruptible power supply (UPS) systems, and selected critical instruments are additionally connected to backup generators to ensure uninterrupted operation and data integrity during power outages.
Laboratory safety regulations and standard operating procedures (SOPs) are prominently displayed on laboratory walls and at main entrances for easy reference by all users. Detailed SOPs for each water and wastewater quality testing parameter are available and strictly followed. Regular safety training sessions, mock drills, and orientation programs are conducted for students and staff to enhance awareness of emergency response, chemical handling, waste management, and laboratory best practices.
Furthermore, laboratories implement proper chemical storage practices, segregation of incompatible chemicals, clearly labeled storage cabinets, and biohazard and chemical waste segregation systems in accordance with EPA and SEPA guidelines. Routine equipment calibration, preventive maintenance, and safety inspections are performed and documented to ensure operational reliability and regulatory compliance.
Laboratory Safety Guidelines and Procedures
The Laboratories in the Environmental Engineering department of USPCASW, Mehran University of Engineering & Technology Jamshoro, provide students with the best opportunity to gain practical knowledge. Students, Staff and Faculty should make themselves aware of the possible hazards present in the laboratory. Students must read and understand the information in this poster about laboratory safety and emergency procedures before the first laboratory session.
Students were advised to follow written and verbal safety instructions throughout the academic terms. Since additional instructions may be given at the beginning of laboratory sessions, all students must arrive at each session on time. Nevertheless, research and teaching workplaces (labs) are full of potential hazards that can cause serious injury and damage to the equipment. Working alone and unsupervised in laboratories is forbidden. With prior approval, at least two people should be present so that one can shut down equipment and call for help in the event of an emergency.
- It is expected that each student will work responsibly.
- Knowing the location of the fire extinguisher beside lab and knowing how to use them.
- Notifying teacher or faculty staff immediately after an injury, fire or explosion, or spill.
- Knowing the building evacuation points and locations of fire extinguisher and first aid kit box.
- It is always better to ask questions than risk harm to oneself and others or damage the equipment.
- The students and faculty's responsibility are to read safety and fire alarm posters and follow the instructions during an emergency.
- If students are not sure how to handle a particular situation, they should ask the Teaching Assistant or Instructor for advice.
General Guidelines on Personal and Laboratory Safety
Think Safety – Work Safely
A. General Laboratory Safety Rules
- Always read chemical labels and equipment instructions carefully before use.
- Never work alone in the laboratory under any circumstances.
- Unauthorized experiments are strictly prohibited.
- Maintain a clean, organized, and clutter-free workspace at all times.
- Do not take shortcuts; strictly follow laboratory rules and SOPs.
- Use only approved tools and equipment and operate them correctly.
- Eating, drinking, smoking, or chewing gum is strictly prohibited in the laboratory.
- Horseplay, running, or distracting behavior is not allowed.
- Report equipment malfunction or failure immediately to the lab assistant or supervisor.
- Immediately report unsafe conditions, such as damaged electrical cables or water leakage.
- Keep books, bags, and personal belongings away from instruments to prevent hazards.
- Do not operate any equipment unless trained and authorized by the lab supervisor.
- Never leave ongoing experiments unattended; if unavoidable, switch off ignition sources and secure the laboratory.
- Clean the work area thoroughly before leaving the laboratory.
B. Personal Protective Equipment (PPE) Requirements
All personnel must wear appropriate PPE before starting any laboratory activity:
- Safety goggles for eye protection
- Laboratory coat (must remain properly fastened)
- Chemical- and heat-resistant gloves
- Face shield (when required)
- Closed-toe shoes (no sandals or open footwear)
- Hair must be tied back
- Long sleeves rolled up
- Remove dangling jewelry
- Never touch or taste any chemical
- Wash hands thoroughly after handling chemicals or samples
C. Laboratory Access, Authorization, and Documentation
- Students working after 5:00 PM or on holidays must submit a request form and obtain approval from the Lab In-Charge.
- All users must record instrument usage (In/Out) in the logbook.
- Field equipment rental (internal or external projects) requires prior approval at least one week in advance.
- Project students must pay equipment rental and analytical charges as per the approved rate list available on the website.
- Before storing samples in refrigerators, students must label samples clearly and log them properly.
D. Chemical Handling and Experimental Safety
- Use a fume hood while working with volatile, flammable, or toxic chemicals.
- Never pour water into acid; always add acid slowly to water.
- Never smell chemicals directly or pipette by mouth.
- Never return unused chemicals to reagent bottles.
- Read the Material Safety Data Sheet (MSDS) before using or storing any chemical.
- Chemicals must be stored according to compatibility and hazard classification.
E. Waste Management and Disposal
- Clean all spills immediately and inform your advisor.
- Dispose of broken or damaged glassware in designated glass bins only.
- Do not use domestic glassware (e.g., drink bottles or jars) in the laboratory.
- Segregate waste properly:
- Broken glass bin (non-hazardous only)
- Contaminated waste bin (hazardous solids: glass, plastic, paper)
- Electronic waste (items with circuitry or batteries)
- Label all waste containers clearly with appropriate waste labels.
- Store waste in designated ventilated areas until collection.
- Handle waste only if trained and aware of associated hazards and PPE requirements.
- Acid-contaminated glassware must be rinsed in a fume hood before disposal.
F. Emergency and Security Measures
- Only RFID-authorized students are permitted access to laboratories.
- In case of emergency, use the internal emergency exit door located near the MS and PhD student sitting area (research fellow room).
- First Aid Box is available in each general laboratory.
- Fire extinguishers are installed in all laboratories.
- Emergency Fire Contact Number: 16
- Do not use mobile phones or attend calls while working in the laboratory.
- Never open doors while wearing gloves to avoid contamination.
- Safety shower include eye wash station are available in labs.