Mr. Asad Ali Laghari


Degree Program

MS - Environmental Engineering

District - Province

Hyderabad, Sindh

Email Address

[email protected]

Identification of Antibiotic Resistant Bacteria in the Drinking Water Sources of Hyderabad City and its Surroundings
  1. To find Antibiotic resistant bacteria present in the waters of Hyderabad City and its surroundings.
  2. To find out the potential health hazards associated with the identified antibiotic resistant bacteria from that water.

Supervisor Name: Dr. Rasool Bux Mahar, Professor, USPCAS-W, MUET, Jamshoro

Co-Supervisor Name: Dr. Zulfiqar Merani

Resistance offered by bacteria to the antibiotics is a global public health issue today. The areas where large quantities of antibiotics are used, greater concentration of antibiotic-resistant bacteria in the water is commonly observed. These bacteria make their way into the aquatic ecosystem quite easily. They also possess the quality of gene transfer. Therefore, they have started to become a threat to the world. In this study, antibiotic-resistant bacteria were identified from different drinking water sources of Hyderabad city and its surroundings. For proper representation of the city, the samples were collected randomly from different drinking water sources including surface water, ground water, tap water, and distribution network.

Antibiotic resistance pattern of bacteria was determined against commonly used antibiotics of the Hyderabad city, using standard Disc Diffusion method. Antibiotic sensitivity and resistivity testing was performed for 62 samples, collected for this study. The most common organisms that were isolated in the samples were: Shigella Species, Pseudomonas Aeruginosa, Vibrio Species, and Escherichia coli. Antibiotic resistance was checked for 16 antibiotics and each sample was seen to resist at least two of the 16 antibiotics. The findings of the study showed that the different drinking water sources in Hyderabad city were contaminated potentially with pathogenic multi-drug resistant strains of different organisms.